Proteomics: the basic overview.

Introduction 2 Deoxyribonucleic Acid to Ribonucleic Acid 4 Ribonucleic Acid to Protein 6 Proteome Handling in Classical and Functional Proteomics 6 Protein Purification 6 Cell Culture 8 Other Source of Samples 8 Subpopulation of Cells 10 Subcellular Components 11 Protein Separation by Two-Dimensional Gel Electrophoresis 12 Principles of Two-Dimensional Gel Electrophoretic Separation 13 Detection of Proteins Separated by Two-Dimensional Gel Electrophoresis 15 Software to Handle Two-Dimensional Gel Electropherogram 17 Potein Processing 17 Blot Digestion of Proteins 18 In-gel Digestion of Proteins 19 Protein Identification 19 Edman Degradation 19